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Seahorse Kits


Seahorse Kits

Advanced metabolic analysis solutions for real-time cellular bioenergetics and mitochondrial function studies.


Fuel Inhibitor Pack

Fuel Inhibitor Pack

Note: Previously sold as Seahorse XF Mito Fuel Flex Test Kit

The Agilent Seahorse Mito Stress Test is the widely recognized, well accepted standard assay for assessing mitochondrial function.

Mitochondrial function Standard assay

Features

Measures basal respiration
ATP-linked respiration
Maximal respiration
Spare respiratory capacity
Non-mitochondrial respiration
Seahorse XF T Cell Metabolic Profiling Kit

Seahorse XF T Cell Metabolic Profiling Kit

Allows robust and accurate measurements of both glycolytic and mitochondrial activities in T and NK cell populations.

T cell analysis NK cell profiling

Features

Quantifies glycolytic and mitochondrial effects
Improved reagents
Reduced assay preparation time
Simplified data analysis
Seahorse XF Mito Tox Assay Kit

Seahorse XF Mito Tox Assay Kit

Allows identification of drug-induced mitochondrial toxicity through direct functional measurements.

Mito toxicity Drug response

Features

High sensitivity and specificity
Distinguishes inhibitors and uncouplers
Improved assay robustness
Automated reporting widgets
Seahorse XF Real-Time ATP Rate Assay Kit

Seahorse XF Real-Time ATP Rate Assay Kit

Quantifies ATP production from glycolysis and mitochondria simultaneously using label-free technology.

ATP production Label-free

Features

Measures ATP production rates
Real-time kinetic data
Live-cell metabolic analysis
Cloud analytics integration
Seahorse XF Substrate Oxidation Stress Test Kits

Seahorse XF Substrate Oxidation Stress Test Kits

Optimized solutions for rapidly measuring cellular substrate oxidation.

Substrate oxidation Multiparametric

Features

Measures glucose, glutamine, and fatty acid oxidation
Real-time multiparametric information
Pathway-specific inhibitors
Customizable assay combinations
Seahorse XF Palmitate Oxidation Stress Test Kit and FAO Substrate

Seahorse XF Palmitate Oxidation Stress Test Kit and FAO Substrate

Designed for fatty acid oxidation studies with functional real-time analysis.

Fatty acid oxidation FAO substrate

Features

Measures long chain fatty acid oxidation
Ready-to-use Palmitate-BSA substrate
No radioactivity required
Functional real-time analysis
Seahorse XF Mito Fuel Flex Test Kit

Seahorse XF Mito Fuel Flex Test Kit

Measures mitochondrial fuel oxidation and evaluates fuel dependency across key pathways.

Fuel oxidation Pathway dependency

Features

Measures mitochondrial fuel oxidation
Uses pathway-specific inhibitors
Evaluates glucose, glutamine, and FAO dependency
Seahorse XF Glycolytic Rate Assay Kit

Seahorse XF Glycolytic Rate Assay Kit

A metabolic assay for measuring glycolytic rate and detecting respiratory shifts in live cells.

Glycolytic rate Metabolic switch

Features

Measures glycolytic rate
Detects metabolic switches
Reports glycolysis vs mitochondrial respiration
Seahorse XF Glycolysis Stress Test Kit

Seahorse XF Glycolysis Stress Test Kit

Provides a functional readout of glycolytic pathway capacity and cellular metabolic demand.

Glycolysis capacity Stress response

Features

Measures glycolytic pathway capacity
Assesses metabolic demand response
Automated report generation
Seahorse XF Hu T Cell Activation Assay Kit

Seahorse XF Hu T Cell Activation Assay Kit

Designed to detect T cell activation within minutes with a simplified live-cell workflow.

T cell activation Kinetic monitoring

Features

Detects T cell activation within minutes
Kinetic monitoring
Injection port compatibility
Simplified workflow
Seahorse XF Plasma Membrane Permeabilizer

Seahorse XF Plasma Membrane Permeabilizer

Supports controlled plasma membrane permeabilization while preserving mitochondrial integrity.

Membrane permeabilization Mitochondrial preservation

Features

Selective plasma membrane permeabilization
Mitochondrial membrane preservation
Minimal optimization required
Seahorse XF Media, Supplements & Calibrant

Seahorse XF Media, Supplements & Calibrant

Optimized assay support products for XF workflows, including media, supplements, and calibrant preparation.

Assay media Calibration support

Features

Optimized XF assay media
No glucose, glutamine, or pyruvate
Pre-adjusted pH 7.4 options
XF cartridge calibration support



Analyzer Compatibility

Each Seahorse XF kit is formulated for specific Agilent Seahorse Analyzer models. Use this guide to confirm which kit matches your instrument before ordering.

Kit / Reagent XF Pro XF Flex XF96 XF24 XFp XF HS Mini
Seahorse XF Mito Stress Test Kits 103015-100 · 103010-100 (XFp)
Seahorse XF 3D Mito Stress Test Kit 103016-100 - - - - -
Seahorse XF T Cell Metabolic Profiling Kit 103772-100 · 103771-100 (XFp) - -
Seahorse XF Mito Tox Assay Kit 103595-100 - - - - -
Seahorse XF Real-Time ATP Rate Assay Kit 103592-100 · 103591-100 (XFp)
Seahorse XF Substrate Oxidation Stress Test Kits 103672-100 · 103673-100 · 103674-100 - -
Seahorse XF Palmitate Oxidation Stress Test Kit & FAO Substrate 103693-100 - -
Seahorse XF Fuel Inhibitor Pack Renamed Previously sold as Seahorse XF Mito Fuel Flex Test Kit 103260-100 · 103270-100 (XFp)
Seahorse XF Glycolytic Rate Assay Kit 103344-100 · 103346-100 (XFp)
Seahorse XF Glycolysis Stress Test Kit 103020-100 · 103017-100 (XFp)
Seahorse XF Hu T Cell Activation Assay Kit 103759-100 · 103766-100 - -
Seahorse XF Plasma Membrane Permeabilizer 102504-100
Seahorse XF Hu T Cell Activation Assay Kit 103759-100 · 103766-100 - -