Agilent BioTek multimode microplate readers bring multiple detection modes together in a single, upgradable instrument — UV-Vis absorbance, fluorescence, and luminescence, with cell imaging available on select models. Proprietary Hybrid Technology combines the sensitivity of filter-based optics with the flexibility of monochromators, so one system can adapt as laboratory workflows evolve.
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Agilent BioTek multimode microplate readers bring multiple detection modes together in a single, upgradable instrument - UV-Vis absorbance, fluorescence, and luminescence, with cell imaging available on select models. Proprietary Hybrid Technology combines the sensitivity of filter-based optics with the flexibility of monochromators, so one system can adapt as laboratory workflows evolve.
An ideal entry-level multimode reader delivering flexible detection modes and simplified workflows for routine laboratory applications.
Its modular architecture allows laboratories to expand capabilities as research requirements evolve.
Designed for laboratories requiring maximum performance, automation, and sensitivity across complex multimode applications.
Combining multimode detection with advanced microscopy, Cytation provides a complete workflow from quantitative assays to cellular imaging in a single instrument.
Ideal for laboratories performing routine microbial growth curve analysis, the LogPhase 600 provides dedicated OD600 performance without the added complexity of fluorescence, luminescence, or imaging capabilities.
| Specification | Synergy LX | Synergy H1 | Synergy Neo2 | Cytation | LogPhase 600 |
|---|---|---|---|---|---|
| Detection modes | UV-Vis · FL · Lum | UV-Vis · FL · Lum | UV-Vis · FL · Lum | UV-Vis · FL · Lum | Absorbance only (dedicated OD600) |
| Cell imaging | - | - | - | Yes, to 60× (Cytation 1/9) | - |
| Optics | Filter + monochromator | Hybrid (filter + mono) | Hybrid, quad monochromators | Hybrid (filter + mono) | LED + dual photodiode (fixed, 560–640 nm) |
| Variable bandwidth | - | 9–50 nm (1 nm) | Variable | 9–50 nm (1 nm) - Cytation 9 only* | - |
| Ratiometric detection | - | - | Dual PMTs – FP, FRET, TR-FRET, BRET | - | - |
| Lasers | - | - | 100 mW 680 nm Alpha + TRF laser | 100 mW 680 nm Alpha - Cytation 9 only* | - |
| Reagent injectors | - | Optional dual | Dual-reagent injector compatible* | Dual-reagent injector compatible (Cytation 1/9)* | - |
| Environmental control | - | To 45/70 °C ("M2" configs), CO₂/O₂* | To 70 °C, CO₂/O₂ | To 45 °C (Cytation 1) / 65 °C (Cytation 9), CO₂/O₂* | To 45 °C, orbital shaking, Condensation Control |
| Microvolume (Take3) | Yes (16/48) | Yes + Take3 Trio | Yes + Take3 Trio | Yes* | - |
| Automation | - | BioStack/automation ready* | BioStack Neo | BioStack/automation ready (Cytation 1/9)* | (4-plate onboard capacity) |
| Software | Gen5 | Gen5 | Gen5 | Gen5 | LogPhase app (dedicated, not Gen5) |
| Choose it for | Everyday multimode assays | Flexible mid-range with injectors | High-throughput & ratiometric | Multimode plus imaging | Dedicated microbial growth curves |
What this range of UV-Vis absorbance readers does across every model - with notes on where individual instruments go further.
Used by leading institutions driving scientific progress across India and globally.
A multimode microplate reader is an instrument that detects biochemical reactions using multiple detection modes, including absorbance, fluorescence, and luminescence. Advanced systems also support time-resolved fluorescence (TRF), TR-FRET, and AlphaLISA/AlphaScreen assays.
Multimode readers support a wide range of applications, including fluorescence, luminescence, absorbance, time-resolved fluorescence (TRF), TR-FRET, fluorescence polarization (FP), and AlphaScreen/AlphaLISA assays used in life science and drug discovery research.
RFU (Relative Fluorescence Units) and RLU (Relative Luminescence Units) are the measurement units for fluorescence and luminescence assays. These values depend on detector sensitivity, excitation intensity, sample volume, and instrument configuration, so they should not be compared across different instruments.
Filter-based optics provide higher sensitivity and rapid wavelength switching for fluorescence, luminescence, FP, and TRF assays. Monochromator-based optics offer flexible wavelength selection across a broad spectral range without changing filters. Hybrid readers combine both technologies for maximum flexibility and performance.
A hybrid multimode reader combines independent filter-based and monochromator-based optical systems in a single instrument. This provides both the sensitivity of filters and the flexibility of monochromators, allowing the instrument to support a broader range of applications.
In certain applications, monochromator optics may offer slightly lower sensitivity because of their longer optical path. However, variable-bandwidth monochromators can improve sensitivity and specificity for multiplex assays by narrowing the wavelength range to isolate individual targets.
Laser light sources provide a stable, high-intensity beam that is essential for demanding assays such as AlphaScreen, AlphaLISA, HTRF, LanthaScreen, and TR-FRET. They deliver greater excitation efficiency and improved assay sensitivity than conventional flash lamps.
Bottom reading detects fluorescence or luminescence through the bottom of the microplate, making it ideal for live-cell assays where cells are attached to the well surface. This approach maximizes sensitivity for applications such as ATP viability, GFP expression, and cell migration studies.
Extended dynamic range allows both weak and strong signals to be measured accurately within the same experiment, reducing the need for sample dilution or repeated measurements. This is particularly valuable for enzyme kinetics, growth curves, and endpoint assays.
Yes. Most Agilent BioTek multimode readers include programmable shaking with adjustable speed and duration. Some models also provide advanced shaking profiles optimized for specific biological applications.
Agilent BioTek offers an optional gas controller that maintains controlled CO₂ (0–20%) and O₂ (1–19%) levels inside compatible plate readers, enabling live-cell assays such as cell proliferation, oxidative stress, hypoxia, and metabolic activity studies.
Reagent injectors are used for assays that require compounds to be added during measurement or involve rapid reaction kinetics, including calcium flux assays, dual-luciferase assays, ion channel studies, enzyme kinetics, and other real-time cellular assays.
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