Multi-color flow cytometry systems designed for immunophenotyping and translational research workflows.
Agilent in India
30+ Years Enabling Indian Labs
Installation, Training, Application & Service Support Across India
Supporting India’s leading research, academic & biopharma institutions
The Agilent NovoCyte range spans a single family - from a budget-friendly benchtop analyzer through four- and five-laser systems to a spectral cytometer - all running the same NovoExpress software. Shared design principles carry across the range: fixed, alignment-free optics, a wide dynamic range that removes per-detector voltage tuning, and volumetric absolute counting without reference beads. Match laser count and detector channels to your panels today, and upgrade as your work grows.
Advanced spectral technology enables confident resolution of highly complex multicolor experiments with simplified workflows.
Designed for laboratories requiring high sensitivity, reliable automation, and effortless multicolor analysis.
Combining flexible configuration with powerful analysis software, Quanteon is ideal for advanced routine flow cytometry applications.
A scalable platform that adapts to expanding research needs while maintaining simple, reliable operation.
An affordable, easy-to-use flow cytometer that delivers dependable performance for everyday research and routine multicolor applications.
A side-by-side view to match the right cytometer to your panels. The Opteon is a spectral system, so its 73 detectors capture the emission spectrum for software unmixing rather than mapping one filter to one dye like the conventional models’ channels. A dash marks a spec that doesn’t apply to that model – spectral unmixing, for instance, is unique to the Opteon.
| Specification | NovoCyte Opteon | NovoCyte Quanteon | NovoCyte Penteon | NovoCyte Advanteon | NovoCyte |
|---|---|---|---|---|---|
| Type | Spectral | Conventional | Conventional | Conventional | Conventional |
| Lasers | 3, 4, or 5 | 5 | 4 | 1, 2, or 3 | 1, 2, or 3 |
| Detection Channels | Up to 73 detectors | Up to 30 channels | Up to 25 channels | Up to 21 channels | Up to 17 parameters |
| Dynamic Range | 7.2 log | 7.2 log | 7.2 log | 7.2 log | 7.2 log |
| Spectral Unmixing | Yes (live) | - | - | - | - |
| Small-Particle Resolution | 0.08 µm (VSSC) | 0.1 µm (100 nm) | 0.1 µm (100 nm) | 0.1 µm (100 nm) | 0.2 µm |
| Absolute Counting | Volumetric | Volumetric | Volumetric | Volumetric | Volumetric |
| Autosampler | NovoSampler S | NovoSampler Q | NovoSampler Q | NovoSampler Q | NovoSampler Pro |
| Software | NovoExpress | NovoExpress | NovoExpress | NovoExpress | NovoExpress |
| Choose it for | High-parameter spectral panels | Demanding 30-color panels | High-parameter 4-laser workflows | Flexible, upgradeable mid-range | Budget-friendly entry & routine assays |
Shared strengths across the NovoCyte family - with notes on where specific models extend them.
Used by leading institutions driving scientific progress across India and globally.
A flow cytometer analyzes individual cells and measures multiple characteristics. A cell sorter performs the same analysis while also physically separating selected cell populations. A cell counter measures cell concentration and viability.
Each laser excites specific fluorochromes, enabling multiple cellular markers to be detected simultaneously. Common laser wavelengths include Violet (405 nm), Blue (488 nm), Yellow-Green (561 nm), and Red (635–640 nm).
Automated systems such as NovoCyte with NovoSampler Q streamline sample loading, acquisition, and analysis with minimal user intervention. They can operate unattended for up to 24 hours, improving reproducibility and reducing hands-on time.
Flow cytometers can analyze PBMCs, whole blood, cultured cells, solid tissue, microbial samples, marine samples, and extracellular vesicles.
Depending on the instrument configuration, NovoCyte systems can measure from 4 to more than 45 parameters in a single experiment.
Advanced NovoCyte flow cytometers can acquire data at rates of up to 100,000 events per second.
Typical supported sample volumes range from 10 µL to 5 mL, depending on the instrument and application.
Spectral flow cytometry captures the full emission spectrum of each fluorochrome, creating a unique spectral fingerprint. This allows more fluorochromes and markers to be measured in a single panel than conventional flow cytometry.
Autofluorescence is treated as its own spectral signature and computationally separated from true fluorescence signals, improving data quality and panel performance.
Spectral flow cytometry supports larger, more complex panels in a single experiment, reducing the need for multiple staining panels and conserving valuable samples.
The number depends on the instrument configuration and fluorochrome panel. A five-laser spectral flow cytometer can theoretically support up to 60 parameters in a single experiment.
Medispec India is the authorized channel partner of Agilent laboratory equipment, providing local sales, installation, certification, and service support.
Built on long-term partnerships, Medispec connects global technology innovators with India’s research, healthcare, and life science ecosystem.
All © Rights Reserved Medispec
A Wing, Unit No – 601,
Lotus Corporate Park, Jay Coach,
Off Western Express Highway,
Goregaon (East),
Mumbai – 400063, India